Hydrophobic interaction chromatography (HIC) is a technique employed for the segregation of macromolecules by exploiting the reversible interaction between the external hydrophobic region of a biological macromolecule and the hydrophobic ligand of a HIC medium, such as phenyl, octyl, or butyl. The interaction is amplified in the presence of a buffer with a high salt concentration, and conversely reduced with a low salt concentration. As a result, based on the salt concentration in the buffer, the protein with less hydrophobicity tends to elute first, whereas the protein with more hydrophobicity elutes last. This method is gaining popularity over other chromatography methods in both analytical and preparatory scale applications, owing to its usage of a less denaturing environment.

The BcMag™ Phenyl Magnetic Beads are distinguished by their homogeneity and superparamagnetic properties. They possess hydrophobic entities on their exterior that expedite their effectiveness as a chromatographic matrix. These beads are meticulously crafted to allow for manual or automatic purification, desalination, and concentration of proteins or peptides within the femtomolar to picomolar range, consequently eliminating the need for repetitive pipetting and centrifugation procedures that can be laborious and time-consuming. Additionally, the BcMag™ Phenyl Magnetic Beads, like C8 beads, share similar polarity; however, they incorporate an electron-rich aromatic ring, which provides exclusive selectivity and retention.

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Bioclone生物磁分离技术已经在生物科学的广泛应用中引起了广泛的兴趣。该
技术的优点是可以快速，简单和灵活地处理大型或微升规模的生物样品，
而无需费力地重复移液和离心操作。Bioclone已开发出广泛的产品组合，包括独特的
高品质超顺磁珠（亲和磁珠，反相磁珠，正相
磁珠，疏水相互作用磁珠，离子交换和混合模式磁珠），可用于
包括分馏在内的各种应用的蛋白质，肽，细胞，细菌，抗体，DNA / RNA和其他分子
复杂的生物样本。这些功能性磁珠（颗粒）与市售
自动磁处理器或简单的台式手动分离器兼容。