BcMag™ Cyanopropyl Magnetic Beads are uniform superparamagnetic resins containing cyanopropyl groups on their surface. The beads are specifically designed for quickly purifying, desalting, and concentrating femtomolar to the picomolar scale of very hydrophobic peptides or proteins that may be irreversibly retained on more hydrophobic C18 beads, manually or automatically without the need for a laborious repeat of pipetting and centrifugation.

Magnetic cyanopropyl (CN) bead is a mildly hydrophobic resin that can be used as a normal or reverse phase adsorbent. Under normal phase conditions, the polar component is removed from the non-polar organic solution. Under reversed-phase conditions, non-polar or weakly polar acid, medium, and basic biomolecules are removed from the aqueous solution. Furthermore, as a complex ligand, the cyanopropyl group can concentrate certain metal ions in an aqueous solution.

Hydrophobic interaction chromatography (HIC)

Hydrophobic interaction chromatography  (HIC) is a technique for separating macromolecules from one another based on a reversible interaction between the external hydrophobic region of a biological macromolecule and the hydrophobic ligand (such as phenyl, octyl, or butyl) of a HIC medium. The interaction is enhanced by a buffer with a high salt concentration and reduced with a low salt concentration. Therefore, based on salt concentration in a buffer, the protein with less hydrophobicity is eluted first, whereas the protein with more hydrophobicity elutes last. Compared with other chromatography methods, HIC is a more popular method for separating and purifying protein and peptides in analytical and preparatory scale applications since it employs a less denaturing environment.

Hydrophobic magnetic beads

Bioclone hydrophobic magnetic resins are designed as uniform magnetic beads grafted with a high density of hydrophobic ligands on the surface. The hydrophobic magnetic beads are rigid polymeric beads with covalent surface chemistries, allowing easier handling and packing while providing more excellent physical and chemical stability—resulting in a robust production process. The beads replace time-consuming, difficult, and expensive chromatographic techniques such as agarose, cellulose, Sepharose, Sephadex-based columns, or resins. The hydrophobic magnetic beads are manufactured using nanometer-scale superparamagnetic iron oxide as core and entirely encapsulated by a high purity silica shell, ensuring no leaching problems with the iron oxide. The pure inert silica makes less nonspecific binding. The beads are much smaller (1 and 5 µm diameter) in size and are non-porous, which exhibit larger surface area, less nonspecific binding, and higher resolution than porous supports.

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MSDS

Bioclone生物磁分离技术已经在生物科学的广泛应用中引起了广泛的兴趣。该
技术的优点是可以快速，简单和灵活地处理大型或微升规模的生物样品，
而无需费力地重复移液和离心操作。Bioclone已开发出广泛的产品组合，包括独特的
高品质超顺磁珠（亲和磁珠，反相磁珠，正相
磁珠，疏水相互作用磁珠，离子交换和混合模式磁珠），可用于
包括分馏在内的各种应用的蛋白质，肽，细胞，细菌，抗体，DNA / RNA和其他分子
复杂的生物样本。这些功能性磁珠（颗粒）与市售
自动磁处理器或简单的台式手动分离器兼容。