Hydrophobic interaction chromatography  (HIC) is a technique for separating macromolecules from one another based on a reversible interaction between the external hydrophobic region of a biological macromolecule and the hydrophobic ligand (such as phenyl, octyl, or butyl) of a HIC medium. The interaction is enhanced by a buffer with a high salt concentration and reduced with a low salt concentration. Therefore, based on salt concentration in a buffer, the protein with less hydrophobicity is eluted first, whereas the protein with more hydrophobicity elutes last. Compared with other chromatography methods, HIC is a more popular method for separating and purifying protein and peptides in analytical and preparatory scale applications since it employs a less denaturing environment.

diPhenyl magnetic beads are highly uniform and superparamagnetic, featuring hydrophobic groups on their surface that make them effective as a chromatographic matrix. They enable manual or automatic purification, desalination, and concentration of peptides or proteins in the femtomolar to picomolar range, eliminating the need for repetitive pipetting and centrifugation procedures. BcMag™ diPhenyl Magnetic Beads are perfect for polypeptides with aromatic side chains, large, hydrophobic proteins, membrane-spanning peptides, lipid peptides, and fusion proteins from inclusion bodies. Choose between diPhenyl magnetic beads or BcMag™ diPhenyl Magnetic Beads for efficient and versatile protein purification.

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Bioclone生物磁分离技术已经在生物科学的广泛应用中引起了广泛的兴趣。该
技术的优点是可以快速，简单和灵活地处理大型或微升规模的生物样品，
而无需费力地重复移液和离心操作。Bioclone已开发出广泛的产品组合，包括独特的
高品质超顺磁珠（亲和磁珠，反相磁珠，正相
磁珠，疏水相互作用磁珠，离子交换和混合模式磁珠），可用于
包括分馏在内的各种应用的蛋白质，肽，细胞，细菌，抗体，DNA / RNA和其他分子
复杂的生物样本。这些功能性磁珠（颗粒）与市售
自动磁处理器或简单的台式手动分离器兼容。